Nov 19, 2016 · I'm trying to understand enzyme kinetics, the formula for Km and Kcat make sense to me. Km , the substrate concentration at which the reaction rate is half of Vmax Kcat, used to …

Jan 31, 2025 · Is there a way to estimate the kcat of an enzyme using a kinetic assay if we don't know the enzyme concentration? I thought that perhaps one could fit the Michaelis-Menten equation to a …

I understand that Kcat (turnover number) = Vmax/total enzyme concentration. However the formula I have been given is Kcat = specific activity/molecular mass of enzyme. What is the relationship bet...

I was wondering what the constants in the Michaelis-Menten equation actually mean in experimental data of enzymes. How do I process the data to find Km and Kcat? I did an experiment on catalase an...

Jul 30, 2020 · Welcome to the Biology community! Aside from grammar and punctuation fixes, your question needs attention to the following issues: 1. Please mention the source of the information …

Aug 22, 2015 · @wswr kr is dissociation rate constant. kcat is the turnover number or the rate constant of product formation. Both these reduce the concentration of the [ES] complex.

Jan 2, 2022 · Another issue which makes me think so: In all the biochemistry literature that I have encountered Km and Kcat appears for each enzyme with no indication of the temperature or pH for …

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Mar 21, 2016 · Any way to estimate the effect? The native parameters are as follows: kcat 0.5 1/min Km 0.6 microM [E0] 5 micro M [S] 60 micro M Could I expect to retain them after immobilization? Or are …

Jul 8, 2020 · This is the graph of the Michaelis-Menten equation which describes the relationship between reaction rate and substrate concentration: I don't understand why it is hyperbolic. Intuitively, …